Mutant libraries were generated using ethylmethanesulphonate (EMS),

**2-aminopurine**, and ultraviolet light.This definition appears very rarely and is found in the following Acronym Finder categories:

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- 2-Bromo-4,5-Dimethoxyphenethylamine (hallucinogen/entactogen)
- Two Channel (combiner type)
- Two-Cascaded Single-Stage Distributed Amplifier
- Two Dimensional
- Two Dimensional Fast Fourier Transform
- Two-Dimensional Gabor Filter Bank
- Two-Dimensional Multi-Resolution Hidden Markov Model
- Two-Dimensional Electromagnetic Bandgap Material
- Two-Dimensional Fast Lattice Recursive Least Squares
- Two-Dimensional Fourier Transform

Mutant libraries were generated using ethylmethanesulphonate (EMS), **2-aminopurine**, and ultraviolet light.

The addition of 5-methylcytosine and **2-aminopurine** adjacent to the deletion improved discrimination dramatically.